FastQCFastQC Report
Tue 30 Jun 2015
PatientXsomatic.0.tumor.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePatientXsomatic.0.tumor.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174508
Sequences flagged as poor quality0
Sequence length251
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTACCCTGATTCCCCAGCTCAGCCCCACCACCTCCACCTCCTCCCCTG10960.6280514360373164No Hit
TGGAATTGGTGTTGATGACCTTCGTCGCTTATGCATACTCAGGATGAGTT7270.41659981204300084No Hit
AAAGCCTGGAGGTGGGGAGGCGGTGCACCAGCGCGGGTGGGAGTGAGAGG5780.3312169069612854No Hit
TTCACCAGCAATGGGAACCTCTTTTCCTCTTCCATATAACATATATGGAA5080.29110413276182184No Hit
CTCCCACTCACAGGGTGCCCAGGGACAAAGCCCATGCACCACTCCGGGCT4520.2590139134022509No Hit
TCTCTACAGATCATCACACCCCACCTACACCAAATAGACTAAAATATTTG4450.25500263598230455No Hit
TAGACTGGCCCACGAAGACTGGTGCCATGATTACCTTCAAGATCTGGACA3640.2085864258372109No Hit
ACTCATTCTTTTCTTTTACCTTTTTCTCTTCCATATAACATATATGGAAG3540.2028560295230018No Hit
ATGCCCCGGGCCACCTGGTAGGCACAGGACACCAGGTCCTTGGAGGAGAG2960.1696197309005891No Hit
TTTCTCATCTGTACCCCACGTCCCCCAGTTCTCAATTTTTGCCTGATGTC2210.1266417585440209No Hit
GCTCCCTCTCAAGGGCAGCCACACAGATCGGCCGCGTCGGGCGCTCACAC2210.1266417585440209No Hit
ATGGAGACACGCACACATGCACACACACACGCGCGAATGCACACTCAACA2130.12205744149265364No Hit
AGACAGACAAAACTCCCTCCCTCCCTTTTTCCAAACATTCTGATTTGGAA2020.11575400554702364No Hit
TTTTACCCTGATTCCCCAGCTCAGCCCCACTCTGGGCCCAGCCTTAGCCC1950.11174272812707728No Hit
CCCCCTGCTGGATTGAGCTGCACCTGAATCCAACAGGGAGAGGGAACAAG1910.10945056960139363No Hit
GCGGATGTGCACACACACACACACACAGATTCAAATATTGCAAGTAAAAG1890.10830449033855181No Hit
TCTAACACTACACAGCTGAGAGGCAGTGGAACTTGGTTCCAAATCCTAAT1890.10830449033855181No Hit
TGTGACCGCAACGTAGGAGGGCGAGCAGAGCCCGGAGCGGAGGCTCGGGG1850.10601233181286819No Hit
CACTTCAGTTGGTGACAGAACACAAAAGGAAAAAATTCCTACGTATACAC1750.1002819354986591No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATTG100.0014765275244.922647
TCGGCAC201.5604382E-7244.922643
CCCGCGT151.5190821E-5244.922647
CGGATGT1900.0232.031982
GACACGC5550.0216.2386
GCGGATG2050.0215.362561
CCGACTG700.0209.93373
CGGCCGT850.0201.990371
TACCCTG5350.0201.431694
CGCGGCG254.7121466E-7196.219221
TATCGGG254.7121466E-7196.219221
AGACACG6000.0195.938115
TTACCGG700.0192.439224
GTTAACG650.0188.402025
GCACGGC600.0183.691975
TACGCGC204.7854184E-5183.691976
GGAGACA6850.0175.200133
GCGGAAA351.2050805E-8174.944756
TGATTCC6100.0174.657949
TAGTGCA850.0172.886575
Produced by FastQC (version 0.11.2)