FastQCFastQC Report
Tue 30 Jun 2015
PatientXsomatic.0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePatientXsomatic.0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229377
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCACCAGCAATGGGAACCTCTTTTCCTCTTCCATATAACATATATGGAA18620.8117640391146452No Hit
ACTCATTCTTTTCTTTTACCTTTTTCTCTTCCATATAACATATATGGAAG16220.7071327988420809No Hit
TCTCTACAGATCATCACACCCCACCTACACCAAATAGACTAAAATATTTG15860.6914381128011963No Hit
AGACAGACAAAACTCCCTCCCTCCCTTTTTCCAAACATTCTGATTTGGAA14740.6426102006739997No Hit
ATATTAAAAACAACTTGAATGCAACATACACACATAACACTACTGTGAAA9800.42724423111297116No Hit
TTTCTCATCTGTACCCCACGTCCCCCAGTTCTCAATTTTTGCCTGATGTC6540.2851201297427379No Hit
AACTTTTTCAATTACCTAAGAACTGTGGCCCCTCAAATATACAGTCTTTA5690.2480632321462047No Hit
ACATCTGCATTTTCACCCCACCCTAAAATCTCAAAAGTTGAGATTTTGGG5410.23585625411440556No Hit
CCTTTAAGGGCATCAGTCCCCAAAATCTCAAAAGTTGAGATTTTGGGGTG4950.21580193306216405No Hit
CCCCCTGCTGGATTGAGCTGCACCTGAATCCAACAGGGAGAGGGAACAAG4760.207518626540586No Hit
GGCACATTATTTTTGAAGTATGTTCAACAATAATGTCCCAGATCGCATTA4240.1848485244815304No Hit
AGAAGTCAGTAGAATAAAATCTTAAAGCACTGATAGCACTTAAACTAGTT3290.14343199187364034No Hit
ACCCCATCTCTTTTCCTTTCTCTTCCATATAACATATATGGAAGAGGAAA3020.13166097734297685No Hit
AGCTTCTGGGTTCAGATGATATAAATGTGGCCCCTCAAATATACAGTCTT3000.13078905034070548No Hit
GGCACATTATTTTTGAAGTATGTTCAACAATAATGTGCAGAGAACCGGCT2650.11553032780095651No Hit
TTTCTGCCAAAATTAATGTGCTGAACTGATTCCTATGGCAATCAGTTCTC2400.10463124027256439No Hit
CGACAAAACCAGTTAGAGTAACCTACACCAAATAGACTAAAATATTTGGT2310.10070756876234323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC201.557255E-7245.05671
ACCGGGG100.001476338244.949846
GCCGCGG100.001476338244.949849
CGCCTAG100.001476338244.949848
CGGTGTC201.5606383E-7244.949845
GCGGATG4200.0239.222031
CGGATGT4400.0231.032262
CGGCCGT950.0219.261261
TCCGGTC400.0214.331129
GTTAACG1450.0211.163685
GACACGC6100.0210.81756
TTACCGG950.0206.273564
TTAACGT1450.0202.717136
GATGTGC4900.0199.959064
CGAGTGG254.7465437E-7195.959887
GGATGTG5150.0192.630463
GTGCACA6000.0191.87747
GCAATGG4550.0191.114738
CGATGAG451.8189894E-12190.516573
ATAGGGA1550.0189.721311
Produced by FastQC (version 0.11.2)