FastQCFastQC Report
Tue 30 Jun 2015
PatientXsomatic.1.tumor.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePatientXsomatic.1.tumor.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174508
Sequences flagged as poor quality0
Sequence length251
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCACAGGGGAGGAGGTGGAGGTGGTGGGGCTGAGCTGGGGAATCAGGGT8240.472184656290829No Hit
TGCTCCCCACCCTTTCACAAAACTCATCCTGAGTATGCATAAGCGACGAA6290.3604419281637518No Hit
CTCTCACTCCCACCCGCGCTGGTGCACCGCCTCCCCACCTCCAGGCTTTA5960.3415316203268618No Hit
CAGCCTTTTCCTCTTCCATATATGTTATATGGAAGAGGAAAAGAGGTTCC4640.26589038897930184No Hit
AGCCAGCCGCCGAGCCCGGAGTGGTGCATGGGCTTTGTCCCTGGGCACCC4100.23494624888257273No Hit
CCCACCTACACCAAATATTTTAGTCTATTTGGTGTAGGTGGGGTGTGATG3710.2125977032571573No Hit
TTACAGTGATGCCCAGCCCCACATCCAGTGGCTAAAGCACATCGAGGTGA3240.18566484058037455No Hit
CAGCCTTTTCCTCTTCCATATATGTTATATGGAAGAGAAAAAGGTAAAAG2740.1570128590093291No Hit
TACAACCCCAGCCACAACCCAGAGGAGCAGCTCTCCTCCAAGGACCTGGT2700.15472070048364545No Hit
CACCAAATGGAAGGACCCCCGCCCAATTTCTCGCCTTTTACTTGCAATAT2390.13695647190959725No Hit
TTACCCTATACATCCACAGGGTTTTGACACTTGTTGTCCAGTTGAAAAAA2180.12492263964975817No Hit
CGGTGTGAGCGCCCGACGCGGCCGATCTGTGTGGCTGCCCTTGAGAGGGA1990.1140348866527609No Hit
GACATCAGGCAAAAATTGAGAACTGGGGGACGTGGGGTACAGATGAGAAA1950.11174272812707728No Hit
CTCCCTCTCTTTTTCCAAATCAGAATGTTTGGAAAAAGGGAGGGAGGGAG1940.11116968849565637No Hit
GTGTGTGCATATGTGTATGTTGAGTGTGCATTCGCGCGTGTGTGTGTGCA1800.10314713365576363No Hit
CCACCCAGCCCCTCCCCGAGCCTCCGCTCCGGGCTCTGCTCGCCCTCCTA1800.10314713365576363No Hit
TGCTTGTTCCCTCTCCCTGTTGGATTCAGGTGCAGCTCAATCCAGCAGGG1760.10085497513007999No Hit
GGAAGGGCGGGGCTAAGGCTGGGCCCAGAGTGGGGCTGAGCTGGGGAATC1750.1002819354986591No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTAGC1000.0208.243673
GTTAGCT1100.0189.312424
CATATGT4900.0182.494458
ACTTCGC950.0180.520834
CACTTCG1100.0178.176393
TTCCGTC351.2030796E-8174.994674
GGGGCTA1700.0172.935919
CGGGGCT1650.0170.752388
GTGCATA4600.0170.42965
CTTCGGC301.1752691E-6163.328376
GGCTCGA301.1752691E-6163.328377
ATCTGCG452.8194336E-10163.328379
ATATGTG5050.0160.094159
GCGGTGC850.0158.52466
GCATATG5150.0156.985527
AATGCGT557.2759576E-12155.904348
ATGCGTC557.2759576E-12155.904349
TCCGCGA402.671186E-8153.120357
TGCATAT5250.0151.662066
CTTCGCA1150.0149.12595
Produced by FastQC (version 0.11.2)