FastQCFastQC Report
Tue 30 Jun 2015
example.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameexample.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229377
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCTTTTCCTCTTCCATATATGTTATATGGAAGAGGAAAAGAGGTTCC17540.7646799809919913No Hit
CTCCCTCTCTTTTTCCAAATCAGAATGTTTGGAAAAAGGGAGGGAGGGAG13260.5780876025059182No Hit
CCCACCTACACCAAATATTTTAGTCTATTTGGTGTAGGTGGGGTGTGATG13040.5684964054809332No Hit
CAGCCTTTTCCTCTTCCATATATGTTATATGGAAGAGAAAAAGGTAAAAG12990.5663165879752547No Hit
TAATGTTCTTTTTCACAGTAGTGTTATGTGTGTATGTTGCATTCAAGTTG8800.3836478809994027No Hit
GACATCAGGCAAAAATTGAGAACTGGGGGACGTGGGGTACAGATGAGAAA5730.24980708615074745No Hit
TTTTCCTAAAGACTGTATATTTGAGGGGCCACAGTTCTTAGGTAATTGAA5160.22495716658601342No Hit
ACCACCCCAAAATCTCAACTTTTGAGATTTTAGGGTGGGGTGAAAATGCA4020.17525732745654535No Hit
TGCTTGTTCCCTCTCCCTGTTGGATTCAGGTGCAGCTCAATCCAGCAGGG3990.17394943695313828No Hit
ACCACCCCAAAATCTCAACTTTTGAGATTTTGGGGACTGATGCCCTTAAA3920.1708976924451885No Hit
GAGGGTTTAATAATGCGATCTGGGACATTATTGTTGAACATACTTCAAAA3550.15476704290316814No Hit
TTTGAAATGAACTAGTTTAAGTGCTATCAGTGCTTTAAGATTTTATTCTA2840.12381363432253452No Hit
CAGCCTTTTCCTCTTCCATATATGTTATATGGAAGAGAAAGGAAAAGAGA2710.11814610880777061No Hit
TTTTCCTAAAGACTGTATATTTGAGGGGCCACATTTATATCATCTGAACC2710.11814610880777061No Hit
ATGACAGCCGGTTCTCTGCACATTATTGTTGAACATACTTCAAAAATAAT2340.10201545926575026No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGCG151.5189586E-5244.952683
CCTGTCG750.0228.622486
CTGTCGG750.0212.292317
ATTCCCG500.0196.304661
CAGCCTT9850.0189.329361
GTCTTTA4000.0187.102871
TTTCCTC11050.0183.99167
GTTGCGA204.7850277E-5183.714514
TCCTCTT11250.0182.8989
TTCCTCT11250.0180.720648
GCCTTTT11100.0176.542473
AGCCTTT11050.0175.124532
CCTTTTC11650.0169.259144
CTAGCCG600.0163.301793
GGTTAGC1350.0163.301773
GTGCATA4600.0159.751745
CTTTTCC12350.0158.673785
CCGTCCG700.0157.469575
TTACGAT557.2759576E-12155.878974
GCATATG5050.0155.217537
Produced by FastQC (version 0.11.2)