FastQCFastQC Report
Wed 1 Jul 2015
father.0.0_phred33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefather.0.0_phred33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000000
Sequences flagged as poor quality0
Sequence length90
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG15100.09.73452684
CGTCTGA12950.09.729156584
ACACGTC16900.08.69771384
CACACGT20050.08.37855984
CACGTCT16350.08.21969982-83
GCACACG22600.07.526104582-83
CGGAAGA39450.06.919744584
GTAGATC6350.0097409116.613783484
TAGATCG15452.1827873E-116.523887684
TCTGAAC15600.06.461157384
AGCACAC27850.06.258158782-83
ATCGGAA43000.06.055457682-83
TGAACTC16852.7126589E-85.981843584
TCGGAAG41500.05.970731784
GTCTGAA15950.05.924415682-83
AGATCGG49350.05.871994584
AGTCACT8650.0046993395.826246384
AACTCCA14903.8639937E-65.637251484
AAGAGCA37800.05.55522884
GATCGGA45400.05.45782884
Produced by FastQC (version 0.11.2)