FastQCFastQC Report
Wed 1 Jul 2015
father.0.1_phred33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefather.0.1_phred33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000000
Sequences flagged as poor quality0
Sequence length90
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG550.005867063519.09178436-37
CCTTCGA2603.4563246E-412.921734
CGTGTAG13500.09.64392284
AGTGTAG5251.5008716E-59.59948184
GTCGTGT16650.09.08058984
TCGTGTA14850.08.62579682-83
CGTCGTG18250.08.51460684
GCGTCGT19900.08.2307184
GTGTAGG15150.08.03916884
GTAGGGA12250.07.54244984
AGCGTCG22100.07.41136382-83
CGGAAGA39100.06.766896784
GAGTGTA5304.069705E-66.73548482-83
AAGAGCG29000.06.516888684
GAGCGTC25200.06.49964882-83
AGAGCGT26700.06.29179480-81
TAGGGAA12300.06.146008582-83
AGATCGG48650.06.04283884
ATCGGAA42450.05.936074782-83
TCGGAAG40400.05.925421784