FastQCFastQC Report
Wed 1 Jul 2015
father.1.0_phred33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefather.1.0_phred33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000000
Sequences flagged as poor quality0
Sequence length90
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT15150.09.98007384
CGTCTGA11600.09.41367584
ACGTCTG13400.09.08943884
ACACGTC16650.09.08096884
CACACGT18850.08.24372382-83
AGCACAC26250.07.359908684
CGGAAGA37900.06.981442584
GCACACG20050.06.912286380-81
TCGGAAG41000.06.86332984
ATCGGAA43300.06.78975484
AAGAGCA36200.06.61317784
TAGATCG15250.06.609753684
AGAGCAC30100.06.55805884
GATCGGA46750.06.1537882-83
GAGCACA29400.06.14278184
AAGATCG16150.05.981349584
AGATCGG50500.05.821563782-83
GAAGAGC38800.05.737041584
GAACTCC14803.4280474E-65.67560584
CAGATCG13601.2732926E-115.404073780-81
Produced by FastQC (version 0.11.2)