FastQCFastQC Report
Wed 1 Jul 2015
father.1.1_phred33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefather.1.1_phred33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000000
Sequences flagged as poor quality0
Sequence length90
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA13750.010.38479384
CGTGTAG12550.09.70456184
AGCGTCG20350.09.28687884
GTGTAGG14200.08.87267484
GTCGTGT15350.08.75514282-83
AAGAGCG29450.08.55633284
CGTCGTG16500.08.39946682-83
AGAGCGT25750.07.665530784
GCGTCGT17650.07.61424680-81
GAGCGTC23150.07.256557584
TAGATCG13950.07.225346684
TCGGAAG40350.07.07761684
ATCGGAA43300.06.983389484
CGGAAGA37750.06.89757484
CTAGATC6801.1165139E-76.48488178-79
TGTAGAT7250.0048965116.372008384
GATCGGA46150.06.279123382-83
AAGATCG18250.06.21330382-83
GTAGGGA12257.0940587E-106.171035384
GTAGATC6951.1296597E-66.042780482-83
Produced by FastQC (version 0.11.2)