FastQCFastQC Report
Wed 1 Jul 2015
mother.0.0_phred33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamemother.0.0_phred33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000000
Sequences flagged as poor quality0
Sequence length90
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC1201.0713848E-921.00050216-17
TACACTC3850.018.5458951
ACACGAC1306.120172E-817.76787814-15
CGATCTA850.004064745714.82240128-29
TACACGA1751.7798229E-613.19899414-15
CACGACG1508.158983E-512.60030116-17
ATCGGAA3905.966889E-611.8452513
AGCGTCG1250.003779278411.75910444-45
GATCGGA3653.9545455E-511.5059722
CTACACG2051.0423368E-511.26743412-13
CGACGCT1400.00875334310.50445318-19
ACACTCT7106.548362E-910.0555722
AGATCGG4302.0816195E-49.7676741
GACGCTC2304.084271E-49.13430618-19
TCGGAAG4856.9522124E-48.6591344
ACTCTTT8752.1187589E-88.6393424
CACTCTT8601.3050048E-67.81335833
TTCCCTA7308.553128E-57.4788829
GACTGGA7501.1934102E-47.2801741
CGGAAGA5950.00519227387.05828575
Produced by FastQC (version 0.11.2)