FastQCFastQC Report
Wed 1 Jul 2015
mother.0.1_phred33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamemother.0.1_phred33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000000
Sequences flagged as poor quality0
Sequence length90
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTC5850.035.179491
ACGACGC1700.027.1764716-17
ACACTCT8700.024.620692
ACACGAC2100.021.014-15
CACTCTT10250.020.0780493
CACGACG2200.020.04545416-17
CTACACG2200.020.04545412-13
TACACGA2100.020.014-15
ACTCTTT9750.019.8153864
CGACGCT2150.019.53488318-19
TTCCCTA6950.015.1079149
TTCCGAT2850.014.73684126-27
CCGATTT1050.001018408314.028-29
TCCGATC2209.426003E-913.36363626-27
GATCGGA3552.0823882E-613.0140852
GACGCTC3250.012.92307818-19
AGATCGG3552.9748702E-511.8309861
CGCTCTT3751.8189894E-1211.7620-21
ACGCTCT3452.910383E-1111.56521820-21
CTCTTTC17600.011.2159085
Produced by FastQC (version 0.11.2)