FastQCFastQC Report
Wed 1 Jul 2015
mother.1.0_phred33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamemother.1.0_phred33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000000
Sequences flagged as poor quality0
Sequence length90
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3551.8189894E-1218.9284842
AGATCGG3905.456968E-1217.2349451
ATCGGAA4102.1100277E-1015.3649663
TCGGAAG4454.5474735E-1115.1002524
CGCCGTG1007.0106785E-414.70797624-25
TACACTC3904.2207466E-712.92620851
ACGACGC1354.7538677E-412.44372716-17
ACACGAC1601.4913085E-411.81181814-15
CGGAAGA5652.536035E-811.1497985
CTACACG1750.0041444949.59944612-13
ACACTCT7107.0132955E-89.4642422
CAGACGT2853.8315298E-48.10479510-11
TCAGACG2600.00138836458.07645710-11
TTCAGAC7351.0763732E-57.9995389
TTCCCTA5450.00220231717.7059779
GTTCAGA6752.833668E-47.4662368
CACTCTT8002.641252E-46.8246063
AGACGTG3450.00292130826.69526612-13
GAGTTCA7700.00127476646.5450776
ACTCTTT7800.00147495036.4611664
Produced by FastQC (version 0.11.2)