FastQCFastQC Report
Wed 1 Jul 2015
mother.1.1_phred33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamemother.1.1_phred33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000000
Sequences flagged as poor quality0
Sequence length90
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTC7200.032.6649361
ACGACGC1450.026.06758516-17
ACTCTTT9300.024.8373934
ACACTCT10000.023.9387322
CACGACG1550.023.03103816-17
CTACACG1900.022.10409212-13
CACTCTT10850.022.0633493
ACACGAC1950.021.53732114-15
TACACGA2050.019.46238314-15
CGACGCT1903.6379788E-1217.68327318-19
GATCGGA4007.2759576E-1216.7991092
AGATCGG4201.8189894E-1115.9991531
ATCGGAA4607.6397555E-1114.6079223
TTCCCTA7100.014.1964319
TCGGAAG4801.5097612E-1013.9992594
GACGCTC2705.456968E-1213.99925918-19
CTCTTTC17950.013.102375
TCCGATC2107.3861884E-813.00061226-27
TTCCGAT2851.72804E-1012.52690526-27
TCTTTCC17350.012.1031066
Produced by FastQC (version 0.11.2)